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| Reagent | Volume (μl) | |
| Mastermix PCR | 10 μl | |
| Primer | ||
| CTX-M IV | forward | 1 μl |
| CTX-M IV | reverse | 1 μl |
| TEM | forward | 1 μl |
| TEM | reverse | 1 μl |
| OXA | forward | 1 μl |
| OXA | reverse | 1 μl |
| SHV | forward | 1 μl |
| SHV | reverse | 1 μl |
| DNA template | 2 μl | |
| Total volume | 20 μl |
