Fig. 1.

Schematic representation of the experimental design. The diagram shows the saxitoxin treatment and analysis during the developmental period (168 hpf) of zebrafish (Danio rerio). Individuals were maintained at 28 °C from 0 hpf until the end of treatment. Treatment was initiated at 5.7–6 hpf when embryos were between the germ-ring and shield stage, lasting until 168 he. Analyses were: 1) Sensorimotor from 48 he; 2) Absolute mRNA quantification from 72 he; and 3) Immunofluorescence assay at 120 and 168 he. Created withBioRender.com.