Fig. 1.
Safety assessment of intravitreal administration of FH535 in C57 mice. A Hematoxylin eosin (HE) staining of the retinas in 7-week and 4-month mice was shown after intravitreal injection of different concentrations of FH535 (0.5 or 3.0 μmol/L) for 7 days. B–E Quantitative analysis of ONL, OPL, INL, and IPL thickness in retinas among abovementioned groups. F–H Representative results of scotopic ERG at 0.01 or 3.0 log cds/m2 and photopic ERG at 3.0 log cds/m2 in 4-month-old male C57BL/6J mice 7 days after intravitreal injection of FH535 (3.0 μmol/L), compared to the Vehicle group injected with PBS. I The mean scotopic ERG b-wave amplitudes elicited by 0.01 log cds/m2 white-light stimuli. J The mean scotopic ERG a-wave amplitudes elicited by 3.0 log cds/m2 white-light stimuli. K The mean scotopic ERG b-wave amplitudes elicited by 3.0 log cds/m2 white-light stimuli. The mean photopic ERG b-wave amplitudes elicited by 3.0 log cds/m2 white-light stimuli. Scale bars, 200 μm. ERG, Electroretinography; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Data are expressed as mean ± SEM. ns, no significant