Table 2.

Differentially expressed genes associated with fruit cuticle biosynthesis

Pepper gene	SGN annotation	Description	A+20	A+30	A+45	Ripe	References	Homologue	Homologue function
CA06G14420	ABC transporter	Putative ABC transporter					Bessire et al. (2011)	At2G26910	ABCG32 full transporter
CA06G14430	Unknown function						Bessire et al., (2011)	At2G26910	ABCG32 full transporter
CA01G00270	Valacyclovir hydrolase	Putative					Kurdyukov et al. (2006)	At4G24140	BDG3 BODYGUARD3
CA01G19070	CD2	Cutin deficient 2					Isaacson et al. (2009)	Solyc01G091630	CD2 CUTIN DEFICIENT2
CA05G18530	Unknown function						Gable et al. (2004)	At3G55360	CER10 ECERIFERUM10 Enoyl-CoA reductase
CA09G18740	CER3	Predicted protein ECERIFERUM 3-like					Rowland et al. (2007)	At5G57800	CER3 ECERIFERUM3 involved in alkane formation
CA11G06990	CER3	Predicted protein ECERIFERUM 3-like					Rowland et al. (2007)	At5G57800	CER3 ECERIFERUM3 involved in alkane formation
CA02G23370	3-ketoacyl-CoA synthase	3-ketoacyl-CoA synthase					Vogg et al. (2004)	At1G68530	CER6 ECERIFERUM6 b-Ketoacyl-CoA synthase
CA05G19790	Cytochrome P450	Cytochrome P450					Li-Beisson et al. (2009)	At3G10570	CYP77A6 CYP77A subfamily
CA08G18140	Predicted long chain acyl-CoA synthetase 2-like	Predicted long chain acyl-CoA synthetase 2-like					Schnurr et al. (2004)	At1G49430	Long chain acyl-CoA synthase
CA08G08360	Predicted long chain acyl-CoA synthetase 4-like	Predicted long chain acyl-CoA synthetase 4-like					Fulda et al. (2002)	At1G64400	Long chain acyl-CoA synthase
CA03G30090	Unknown function						Kranz et al. (1998)	At3G28910	MYB30 MYB transcription factor
CA02G28250	Transcription factor	Transcription factor					Kranz et al. (1998)	At3G28910	MYB30 MYB transcription factor
CA03G30090	Unknown function						Stracke et al. (2001)	At5G62470	MYB96 MYB transcription factor
CA02G28250	Transcription factor	Transcription factor					Stracke et al. (2001)	At5G62470	MYB96 MYB transcription factor
CA10G12030	LTP	Lipid transfer protein					Popovsky-Sarid et al. (2017)
CA10G12060	LTP	Lipid transfer protein
CA09G18740	CER1	Fatty acid hydroxylase
CA12G22670	CER1	Fatty acid hydroxylase
CA11G14620	MYB16	MYB-related transcription factor
CA02G20380	LCFL	Long chain fatty acid CoA ligase
CA03G16520	ERF	Ethylene responsive factor 2A
CA02G17970	PE	Pectinesterase
CA07G11250	ACCOXIDASE	Fruit ripening
CA10G16170	ACCOXIDASE	Fruit ripening
CA10G10710	LTP	Lipid transfer protein
CA10G10770	LTP	Lipid transfer protein
CA10G18900	CYP96A/MAH1	Cuticle development
CA10G18910	CYP96A/MAH1	Cuticle development
CA10G08490	LTP	Lipid transfer protein
CA10G18310	CYTB5	Cuticle development

Red, down-regulated R3; blue, up-regulated R3. Transcript number was assessed at four fruit development stages: anthesis +20 d, anthesis +30 d, anthesis +45 d, and ripe. Genes were identified based on literature searches and using BLAST to identify gene orthologues. Sol Genomic Network gene descriptions are noted, if provided. Genes were considered to be significantly differentially expressed if P<0.05, FDR<0.05. The biological replication is described in the Materials and methods.