Figure 3.

Semen quality parameters analyzed post-thaw between years and thawing methods (Year 0: light blue bar; Year 10, 37 °C: blue bar; Year 10, 70 °C: dark blue bar; n = 9) in Level 1. (A): normal acrosomal ridge (NAR, %) and sperm with intact plasma membrane (SIPM, %), (B): total motile sperm (TMS, %) and progressive motility sperm (PMS, %), (C): spermatozoa with fragmented DNA (FRAG, %). Data are depicted as Means ± SEM. Different lowercase letters indicate differences among groups (p < 0.05).