Table 1.
Kinematic parameters of motility analyzed post-thaw between levels (Level 1 (n = 9), Level 2 (n = 17), and Level 3 (n = 27)) in year 0 and year 10 thawing at 37 °C and in year 10 thawing at 70 °C.
| Parameters | VCL | VSL | VAP | LIN | STR | WOB | ALH | BCF |
|---|---|---|---|---|---|---|---|---|
| Year 0 | ||||||||
| Level 1 | 112.7 ± 4.9 | 99.3 ± 3.4 | 99.3 ± 4.3 | 75.9 ± 1.8 | 86.0 ± 1.5 | 88.1 ± 0.8 | 3.0 ± 0.2 | 8.0 ± 0.2 |
| Level 2 | 98.8 ± 4.4 | 86.0 ± 4.0 | 86.0 ± 3.9 | 71.9 ± 2.0 | 82.5 ± 2.0 | 87.0 ± 0.8 | 2.7 ± 0.1 | 7.6 ± 0.2 |
| Level 3 | 99.1 ± 4 | 87.0 ± 2.9 | 87.0 ± 3.4 | 72.4 ± 1.8 | 82.2 ± 1.7 | 88.0 ± 0.7 | 2.7 ± 0.1 | 7.6 ± 0.1 |
| p-value | 0.18 | 0.05 | 0.14 | 0.52 | 0.5 | 0.6 | 0.3 | 0.3 |
| Year 10 (37 °C) | ||||||||
| Level 1 | 95.2 ± 1.5 | 54.8 ± 2.2 | 78.0 ± 1.3 | 57.8 ± 2.8 | 70.4 ± 3.0 | 81.9 ± 1.0 | 3.1 ± 0.1 | 7.9 ± 0.2 |
| Level 2 | 84.2 ± 2.6 | 50.0 ± 1.5 | 69.0 ± 2.1 | 60.0 ± 2.0 | 73.0 ± 2.1 | 82.1 ± 1.0 | 2.8 ± 0.1 | 7.7 ± 0.2 |
| Level 3 | 83.9 ± 2.8 | 50.9 ± 1.3 | 69.4 ± 2.4 | 61.6 ± 1.7 | 74.6 ± 1.9 | 82.6 ± 0.9 | 2.8 ± 0.1 | 8.0 ± 0.1 |
| p-value | 0.05 | 0.18 | 0.08 | 0.49 | 0.5 | 0.87 | 0.09 | 0.29 |
| Year 10 (70 °C) | ||||||||
| Level 1 | 93.9 ± 3.8 | 54.7 ± 2.0 | 74.9 ± 3.2 | 58.7 ± 2.3 | 73.7 ± 2.7 | 79.7 ± 1.0 | 3.2 ± 0.1 | 7.5 ± 0.2 |
| Level 2 | 89.9 ± 2.7 | 51.6 ± 1.7 | 72.1 ± 2.3 | 57.6 ± 1.6 | 71.8 ± 1.7 | 80.1 ± 0.9 | 3.1 ± 0.1 | 7.6 ± 0.1 |
| Level 3 | 90.3 ± 2.9 | 54.2 ± 1.4 | 73.3 ± 2.4 | 60.6 ± 1.4 | 74.7 ± 1.4 | 81.2 ± 0.6 | 3.0 ± 0.1 | 7.7 ± 0.1 |
| p-value | 0.73 | 0.41 | 0.81 | 0.36 | 0.48 | 0.43 | 0.45 | 0.77 |
Curvilinear velocity (VCL, µm/s), straight-line velocity (VSL, µm/s), average path velocity (VAP, µm/s), percentage of linearity (LIN, %), percentage of straightness (STR, %), wobble coefficient (WOB, %), mean amplitude of lateral head displacement (ALH, mm), and mean beat cross frequency (BCF, Hz). Data are depicted as means ± SEM.