Figure 2. RAGE elevation in murine NASH is enriched on recruited macrophages.

(A) Representative co-IF images of liver cryosections from FFC mice (n = 6) compared with chow mice (n = 5), stained for DAPI, F4/80, and RAGE. A negative control is shown. Scale bar: 50 μm. (B) Schematic depicting experimental design for isolating IHLs for flow cytometry from FFC mice. (C) Quantification of RAGE⁺ among F4/80^–CD45⁺ (nonmacrophages [NonMacs]) and F4/80⁺CD45⁺ (macrophages [Macs]) cells in livers from chow and FFC mice (n = 4 chow, n = 5 FFC, P < 0.01). Cell numbers are presented as cell count per gram of liver. Mann-Whitney U test was used for statistical analyses, and P values were adjusted by the Benjamini-Hochberg method for multiple comparisons. (D) Representative flow cytometry gating demonstrating RAGE expression, depicted as a heatmap of RAGE expression intensity, among recruited (CD11b^hiF4/80^int) and resident macrophages (CD11b^intF4/80^hi) in chow and FFC mice. (E) Quantification of RAGE⁺ recruited macrophages in chow (n = 5) and FFC (n = 6) mice; P < 0.05. (F) Quantification of RAGE⁺ resident macrophages in chow (n = 5) and FFC (n = 6) mice; P < 0.05. Cell numbers are presented as cell count per gram of liver. Mann-Whitney U test was used for statistical analyses, and P values were adjusted by the Benjamini-Hochberg method for multiple comparisons.