Table 2.

Primers and probes were designed to detect plasmid-mediated colistin resistance genes (mcr-1 to mcr-10).

Tube	Target	Primers / Probes name	Sequence (5′-3′)	Length (bp)	Tm (°C)	Final concentration (nmol/L)	Reference
A	mcr-1 mcr-2 mcr-6	mcr-1/2/6-F1	CTGGGCGCGGATGAGTAT	62	58.8	200	This study
		mcr-1/2/6-F2	CTGGGTCAAGATGACTAT		49.7	200
		mcr-1/2/6-R	AGCGTATCH*AGCACATTTTCTTG		58.8	200
		mcr-1/2/6-P	FAM-ATGTCGATACCGCCAAA-MGB		68	100
	16S rRNA	16S rRNA-F	TGGAGCATGTGGTTTAATTCGA	159	59.1	200	Control CfD Prevention
		16S rRNA-R	TGCGGGACTTAACCCAACA		58.6	200
		16S rRNA-P	HEX-CACGAGCTGACGACAR*CCATGCA-BHQ		72	200
B	mcr-3	mcr-3-F	GTGATGATGCAAAACGGGATACT	109	58.9	200	This study
		mcr-3-R	TCTTTCTCATAGCCATTCATCGAG		58.5	200
		mcr-3-P	HEX-AGTAAGCCCACGTTGATGT-MGB		70	200
	mcr-9 mcr-10	mcr-9/10-F	CCCACGCTGATGTTCCTG	86	57.1	200	This study
		mcr-9/10-R	AAGGCATTK*GTR*TCACGCG		55.5	200
		mcr-9/10-P	FAM-ATTGGCGAAACGGCAC-MGB		69	100
C	mcr-4	mcr-4-F	TGCGTTGTTTTATGTTGCCATT	68	59	200	This study
		mcr-4-R	GTTTTTCAATTCCTTTTCGCACTAT		58	200
		mcr-4-P	FAM-TCAATCTACCGCTCTTT-MGB		68	100
	mcr-7	mcr-7-F	CGCACTCAACGCCGTCTT	103	59.6	200	This study
		mcr-7-R	GAGCATGGCGTAACTGACGAT		58.8	200
		mcr-7-P	HEX-CTTCCGCTGGTTGCT-MGB		69	200
D	mcr-5	mcr-5-F	GCAGACGAAGCAAGGGAAGTC	95	60	200	This study
		mcr-5-R	TCCCCGACAACCAGTACGA		58.5	200
		mcr-5-P	FAM-CCGCCGTCCTCGTG-MGB		69	100
	mcr-8	mcr-8-F	CGTTCCTTTTCTTTCCACTCTATTTC	84	58.8	200	This study
		mcr-8-R	CCCCGCTTATTGTCTGTTGAA		58.7	200
		mcr-8-P	HEX-TGTACCAGCAATTATCCT-MGB		69	200

^*R = A or G; K = G, T; H = A, C, T.