Table 3.

Reproducibility assay of TaqMan real-time PCR.

Plasmid (copies)	mcr-1/2/6						mcr-9/10
	Intra-			Inter-			Intra-			Inter-
	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV
107	19.12	0.02	0.12%	19.05	0.07	0.38%	18.39	0.14	0.79%	18.08	0.26	1.46%
106	22.09	0.04	0.16%	22.09	0.08	0.35%	21.18	0.04	0.19%	21.00	0.16	0.76%
105	25.07	0.06	0.23%	25.19	0.09	0.36%	24.23	0.04	0.16%	24.12	0.10	0.42%
104	28.32	0.06	0.20%	28.47	0.11	0.37%	27.41	0.09	0.34%	27.47	0.14	0.50%
103	31.84	0.16	0.51%	31.82	0.03	0.10%	30.82	0.29	0.95%	30.79	0.04	0.14%

Plasmid (copies)	mcr-3						mcr-4
	Intra-			Inter-			Intra-			Inter-
	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV
107	18.34	0.07	0.36%	18.47	0.15	0.83%	19.37	0.06	0.31%	19.29	0.09	0.49%
106	21.48	0.06	0.26%	21.70	0.32	1.46%	21.10	0.20	0.94%	21.05	0.31	1.46%
105	24.58	0.15	0.59%	24.83	0.32	1.30%	24.49	0.07	0.29%	24.44	0.10	0.40%
104	27.99	0.20	0.71%	28.12	0.24	0.85%	27.38	0.18	0.64%	27.44	0.24	0.86%
103	31.20	0.38	1.22%	31.36	0.38	1.22%	31.06	0.07	0.23%	30.93	0.26	0.84%

Plasmid (copies)	mcr-5						mcr-7
	Intra-			Inter-			Intra-			Inter-
	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV
107	19.29	0.06	0.29%	18.87	0.36	1.91%	18.9967	0.18824	0.99%	19.05	0.06	0.31%
106	21.80	0.05	0.21%	21.60	0.18	0.83%	21.1633	0.09074	0.43%	20.83	0.29	1.41%
105	24.88	0.11	0.44%	24.85	0.16	0.63%	23.8133	0.31943	1.34%	23.81	0.08	0.35%
104	28.13	0.04	0.13%	28.16	0.08	0.28%	27.1333	0.07234	0.27%	27.12	0.03	0.11%
103	31.21	0.14	0.43%	31.34	0.17	0.54%	30.64	0.07	0.23%	30.60	0.10	0.32%

Plasmid (copies)	mcr-8						16S rRNA
	Intra-			Inter-			Intra-			Inter-
	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV
107	18.33	0.13	0.71%	18.24	0.12	0.64%	18.9967	0.18824	0.99%	19.05	0.06	0.31%
106	21.43	0.08	0.35%	21.45	0.15	0.70%	21.1633	0.09074	0.43%	20.83	0.29	1.41%
105	24.81	0.07	0.28%	24.75	0.20	0.79%	23.8133	0.31943	1.34%	23.81	0.08	0.35%
104	28.22	0.13	0.46%	28.12	0.11	0.37%	27.1333	0.07234	0.27%	27.12	0.03	0.11%
103	31.24	0.14	0.46%	31.30	0.24	0.76%	30.64	0.07	0.23%	30.60	0.10	0.32%