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. 2024 Feb 22;9(4):e164188. doi: 10.1172/jci.insight.164188

Figure 2. Lung tumor suppression by immune, but not nonimmune, NF-κB2.

Figure 2

(A) Bone marrow transplantation assays showing lung tumor suppression by immune, but not nonimmune, NF-κB2. NF-κB2 KO was confirmed by IB analysis of the spleen from the donor mice. Sample number (n) is indicated at the bottom for each group. (B) Cell growth assays showing no effect of ectopic NF-κB2 on human lung cancer cell growth in culture (n = 6). Ectopic NF-κB2 (p100) expression was confirmed by IB analysis. (C) Soft agar colony formation assays showing no effect of ectopic NF-κB2 on anchorage-independent growth of H727 (n = 5) and H460 (n = 4) human lung cancer cells. Scale bars: 200 μm. (D) TCGA data showing no change in NF-κB2 expression in human lung cancers. (E) Kaplan-Meier survival analysis showing no association of NF-κB2 expression in lung tumors with patient survival. Data are presented as mean ± SEM. *P < 0.05 by ordinary 1-way ANOVA (A), 2-tailed, unpaired Student’s t test (B–D), or log-rank test (E). NS, not statistically significant.