Table 6.
Sample compositions used for calcein release measurements.
Sample | Vpep (µL) | VLUVs (µL) | Vbuffer (µL) | [LyeTx I mn∆K] (µM) |
---|---|---|---|---|
1 | 0 | 150 | 150 | 0 |
2 | 5 | 150 | 145 | 4,2 |
3 | 10 | 150 | 140 | 8,3 |
4 | 20 | 150 | 130 | 16,7 |
5 | 40 | 150 | 110 | 33,3 |
6 | 80 | 150 | 70 | 66,7 |
Vpep = volume of 2 mM peptide stock solution, VLUVs = volume of 200 μM POPC: POPG LUVs stock solution, and Vbuffer = volume of 10 mM Tri-HCl buffer solution (pH 7.4).