FIG. 1.

Recognition of UL45-2 by LSU32 antiserum. CV1 cells were infected with HSV-2 strain 333 at an MOI of 1 PFU per cell (lane 1) or mock infected (lane 2). COS7 cells were transfected with pAC265 by the calcium phosphate procedure (lane 3) or mock transfected (lane 4). Cytoplasmic extracts were prepared at 18 h p.i. or 40 h posttransfection, electrophoresed on a 12.5% denaturing polyacrylamide gel, transferred to nitrocellulose, and probed with LSU32 and then ¹²⁵I-protein A. Molecular weights (in thousands [K]) are noted at the right.