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Time course of UL45-2 synthesis, compared to those of gB-2, gC-2, and gD-2. CV1 cells were infected with HSV-2 at an MOI of 1 PFU per cell. Cytoplasmic extracts were prepared at 2-h intervals from 0 to 12 h p.i., electrophoresed on denaturing polyacrylamide gels, transferred to nitrocellulose, and probed with LSU32 (UL45-2), R90 (gB-2), R46 (gC-2), or R7 (gD-2) and then with 125I-protein A.
