Figure 3.

Silybin enhanced hepatic antioxidant capacity by activating the Nrf2 signaling pathway. The expression of Nrf2 signaling pathway genes including CAT (A), SOD1 (B), GPX1 (C), and GPX4 (D). (E–G) The relative protein expression level of Nrf2 and Keap1. Ctrl, piglets were given a basal diet and were challenged with saline; Si, piglets were given a silybin-supplemented diet and were challenged with saline; PQ, piglets were given a basal diet and were challenged with paraquat; Si + PQ, piglets were given a silybin-supplemented diet and were challenged with paraquat; CAT, catalase; SOD, superoxide dismutase; GPx, glutathione peroxidase; Nrf2, nuclear factor-erythroid 2-related factor 2; Keap1, kelch-like ECH-associated protein l. Data are expressed as mean ± standard error (n = 6). * p < 0.05, ** p < 0.01.