Figure 4.

HLP_B induces significantly greater latency reversal in tCHI samples than in tAHI samples. (A) Viral copies in culture supernatant from latency reversal assay were measured by qRT-PCR. (B) Activation of CD4⁺ T cells was determined by SFU count via IFN-γ ELISpot assay. (C) The ratio of HIV vRNA copies per IFN-γ activated cell was calculated. Each datapoint represents one donor from the respective cohort. (D) Comparison of three HLP formulations (HLP_B, _D, and _A + D) on latency reversal efficiency in the tCHI cohort and intra-donor pairwise alignment comparison among three HLP formulations and CTF was shown. Each pair of datapoints connected by a line is from one donor. Two-tailed Mann–Whitney tests were performed to test inter-sample significance, and two-tailed Wilcoxon matched-pairs signed rank tests were performed to test intra-sample significance. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. HLP, HIV-1/virus-like particle; SFU, spot forming units; L.O.D, limit of detection. Geometric mean shown for scatter, median is shown for box–whisker plots.