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. 2024 Jan 12;11(3):uhae015. doi: 10.1093/hr/uhae015

Figure 1.

Figure 1

Early spur development of Impatiens uliginosa. Paraffin sectioning (longitudinal and transverse sections) was performed on the buds of five consecutive phases to observe the internal structure of spurs, and SEM was used to observe their external morphology. (A–C) Longitudinal section (A), transverse section (B), and SEM (C) of flower buds in phase 1; spurs and ovules have not developed. (D–F) Longitudinal section (D), transverse section (E), and SEM (F) of flower buds in phase 2; ovules can be observed, and spurs are still undeveloped. (G–I) Longitudinal section (G), transverse section (H), and SEM (I) of flower buds in phase 3; spurs are ready to develop, with deep staining (G) and eminence (I) at the growth point. (J–L) Longitudinal section (J), transverse section (K), and SEM (L) of flower buds in phase 4; the primordial spurs extend from the labellum. (M–O) Longitudinal section (M), transverse section (N), and SEM (O) of flower buds in phase 5; spurs extend to about 0.4 mm, forming a distinct intracavity and vascular bundles and growing toward the tip of the bud. Arrows indicate spurs or their growth points. la, labellum; ou, ovule; ov, ovary; p, pollen; pl, placenta; s, sepal; sp, spur; v, vexillum; vb, vascular bundle; w, wing. Bars = 500 μm.