FIG. 5.

Intracellular distribution of viral immunoreactivity in the perirhinal cortex 45 h after injection of PRV-Becker into the PFC (A) and 36 h after identical intracerebral injection of an equivalent concentration of PRV-Bartha (B). Viral antigens were identified with a rabbit polyclonal antibody generated against acetone-inactivated virus. The same dilution and period of incubation of tissue in the primary antibody was used in both cases, and the tissues were processed simultaneously. Note that immunoperoxidase reaction product is confined to the soma and primary dendrites of PRV-Becker-infected pyramidal neurons (A) whereas cells infected with PRV-Bartha exhibit a more extensive intracellular distribution of viral antigen, even though the animal was sacrificed 9 h earlier than the PRV-Becker-infected rat. Bars, 40 μm.