Figure 5.

Production and characterization of a tubular urethra produced in the laboratory by tissue engineering with dermal fibroblasts. (A) The macroscopic appearance of the tubular tissue was reconstructed after rolling a stromal sheet around a mandrel. (B) After urothelial seeding, the tubular tissue is cannulated and put into a bioreactor for maturation. (C) Histological characterization of the tubular tissue after maturation (Masson’s trichrome staining): a well-differentiated urothelium is visible with basal intermediate umbrella cells.