Table 1.

Comparison of the content of GSLs of ER and WR after foliar application of the combination of different elicitors and high temperatures.

Edible Radish
Treatments		Glucosinolates (mg 100 g −1 DW)
Elicitor	Temperature	GRE	4-HGB	DER	GB	MGB	TGLs
C1	20 °C	32.17 ± 7.016 cd	5.20 ± 0.928 bc	7.52 ± 0.627 ab	2.93 ± 0.473 e	3.19 ± 0.545 a	51.01 ± 9.588 de
C2	30 °C	34.92 ± 8.018 cd	4.92 ± 0.589 bcd	8.49 ± 0.990 a	4.41 ± 0.285 d	0.78 ± 0.303 c	53.44 ± 9.233 de
Citric acid	20 °C	34.56 ± 3.285 cd	5.17 ± 0.918 bc	5.34 ± 0.147 d	2.85 ± 0.377 e	2.81 ± 0.115 a	50.72 ± 4.842 de
Citric acid	30 °C	43.38 ± 3.421 bc	3.96 ± 0.091 cd	7.19 ± 0.743 abc	2.46 ± 0.324 e	1.56 ± 0.212 bc	58.55 ± 4.791 cd
MeJa	20 °C	41.05 ± 4.536 bc	3.01 ± 0.793 d	7.63 ± 0.499 ab	8.03 ± 0.585 b	1.85 ± 0.312 ab	61.56 ± 6.725 bcd
MeJa	30 °C	43.45 ± 3.595 bc	7.84 ± 0.969 a	6.58 ± 0.418 bcd	10.22 ± 0.400 a	1.01 ± 0.325 c	69.10 ± 5.707 abc
Chitosan	20 °C	26.69 ± 3.540 d	3.44 ± 1.189 cd	5.35 ± 0.501 d	2.09 ± 0.408 e	2.418 ± 0.719 ab	39.98 ± 6.358 e
Chitosan	30 °C	49.92 ± 8.414 b	3.40 ± 0.581 cd	7.15 ± 0.812 abc	2.18 ± 0.190 e	1.57 ± 0.267 bc	64.24 ± 6.765 bcd
K2SO4	20 °C	54.243 ± 2.804 ab	3.05 ± 0.766 d	8.03 ± 0.611 ab	6.29 ± 0.931 c	2.46 ± 0.300 ab	74.08 ± 5.412 ab
K2SO4	30 °C	64.43 ± 3.062 a	6.78 ± 1.017 ab	5.83 ± 0.262 cd	2.25 ± 0.312 e	2.47 ± 0.251 bc	81.77 ± 4.904 a
Significance
Temperature (A)		***	***	NS	NS	***	***
Elicitor (B)		***	**	***	***	***	***
A × B		**	***	***	***	***	*
Wild Radish
Treatments		Glucosinolates (mg 100 g−1 DW)
Elicitor	Temperature	GRE	4-HGB	DER	GB	MGB	TGLs
C1	20 °C	46.56 ± 0.265 def	0.95 ± 0.239 d	2.52 ± 0.252 c	2.50 ± 0.565 e	0.73 ± 0.404 d	53.27 ± 1.705 d
C2	30 °C	36.17 ± 6.361 f	0.86 ± 0.093 d	1.13 ± 0.272 d	5.33 ± 0.173 cd	1.33 ± 0.231 cd	44.82 ± 7.113 d
Citric acid	20 °C	57.16 ± 5.697 bcd	3.57 ± 0.722 a	1.53 ± 0.601 d	5.93 ± 0.382 c	1.22 ± 0.355 cd	69.42 ± 7.740 c
Citric acid	30 °C	41.92 ± 3.859 ef	1.07 ± 0.510 d	4.58 ± 0.330 a	2.98 ± 0.746 de	0.92 ± 0.304 cd	51.47 ± 5.732 d
MeJa	20 °C	54.65 ± 2.474 cde	1.94 ± 0.520 cd	4.43 ± 0.129 a	17.64 ± 0.876 a	1.74 ± 0.643 bcd	80.01 ± 4.873 bc
MeJa	30 °C	50.97 ± 7.603 cde	1.45 ± 0.680 cd	4.18 ± 0.229 ab	16.41 ± 0.876 a	1.56 ± 0.613 bcd	74.57 ± 9.002 bc
Chitosan	20 °C	60.88 ± 5.338 bc	1.25 ± 0.360 cd	2.61 ± 0.092 c	5.67 ± 1.073 cd	2.90 ± 0.603 a	73.30 ± 7.453 c
Chitosan	30 °C	60.81 ± 5.547 bc	3.83 ± 0.447 a	2.71 ± 0.566 c	4.08 ± 0.336 cde	1.49 ± 0.502 bcd	72.92 ± 7.372 c
K2SO4	20 °C	89.22 ± 3.996 a	3.37 ± 0.327 ab	3.45 ± 0.415 bc	4.92 ± 0.927 cde	2.59 ± 0.436 ab	103.54 ± 6.073 a
K2SO4	30 °C	68.60 ± 6.657 b	2.31 ± 0.273 bc	3.70 ± 0.414 ab	12.37 ± 2.051 b	2.03 ± 0.592 abc	89.01 ± 9.988 ab
Significance
Temperature (A)		***	*	**	*	**	***
Elicitor (B)		***	***	***	***	***	***
A × B		**	***	***	***	**	*

C₁: represent control 1 (20 °C without elicitor) and C₂ represent control 2 (30 °C without elicitor). Mean separation within a column followed by different letters are significantly different according to Tukey test at p ≤ 0.05. NS, *, **, and *** indicate non-significant, significant at p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001, respectively. GRE is glucoraphenin; 4-HGB is hydroxyglucobrassycin; DER is dehydroerucin; GB is glucobrassycin; MGB is 4-methoxyglucobrassycin.