Table 1.

Histology assessing wound bed before grafting. A veterinary pathologist blinded to the groups scored the sections on day 7 for the stated parameters. The 2 groups were compared by performing the nonparametric Mann–Whitney test to compare ranks for statistical significance. The p value was determined for each parameter comparing the allograft wounds to the IFSG-treated wounds, with ‘*’ notating when the value is less than 0.05 and therefore statistically significant. IFSG = intact fish skin graft; IC = inflammatory cell.

DAY 7 ANALYSIS		Allograft (N =12)		IFSG (N = 24)		p Value
Parameter	Pathologist Scoring Rubric	N	%	N	%
Angiogenesis	0—None	6	50	3	12.5	<0.0001 *
	1—≤50 Vessels	6	50	3	12.5
	2—51–100 Vessels	0	0	12	50
	3—101–150 Vessels	0	0	6	25
Foreign Material	0—Absent	6	50	5	20.8	0.1245
	1—Present	6	50	19	79.2
Hemorrhage	0—Absent	6	50	4	16.7	0.0532
	1—Present	6	50	20	83.3
Hemorrhage Severity	0—Absent	6	50	4	16.7	0.2493
	1—Mild	2	16.7	12	50
	2—Moderate	4	33.3	7	29.2
	3—Severe	0	0	1	4.2
Granulation Tissue	0—Absent	10	83.3	4	16.7	0.0002 *
	1—Present	2	16.7	20	83.3
Fibroplasia	0—None	1	8.3	0	0	0.0006 *
	1—Minimal	2	16.7	1	4.2
	2—Mild	6	50	4	16.7
	3—Moderate	3	25	11	45.7
	4—Marked	0	0	7	29.2
	5—Severe	0	0	1	4.2
Neutrophils	0—None	4	33.3	0	0	<0.0001 *
	1—Minimal number of ICs	7	58.3	2	8.3
	2—Mild number of ICs	0	0	13	54.2
	3—Moderate number of ICs	0	0	2	8.3
	4—Marked number of ICs	0	0	3	12.5
	5—Severe number of ICs	1	8.4	4	16.7
Eosinophils	0—None	7	58.3	1	4.2	<0.0001
	1—Minimal number of ICs	4	33.3	2	8.3
	2—Mild number of ICs	1	8.3	12	50
	3—Moderate number of ICs	0	0	9	37.5
Lymphocytes	0—None	4	33.3	1	4.2	<0.0001 *
	1—Minimal number of ICs	5	41.7	0	0
	2—Mild number of ICs	3	25	17	70.8
	3—Moderate number of ICs	0	0	6	25
Macrophages	0—None	6	50	1	4.2	0.0048 *
	1—Minimal number of ICs	5	41.7	16	66.7
	2—Mild number of ICs	1	8.3	7	29.2