Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Feb 23;12(3):507. doi: 10.3390/biomedicines12030507

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescent micrographs of the PDAC-TEMs following single 5 min freeze. At 24 h (A) and 72 h (B) post-freeze, the extent of PANC-1 cell death in TEMs was evaluated via fluorescence microscopy following staining with Calcein-AM (green, live) and propidium iodide (red, dead). Using the Zeiss ZEN software, measurements were made, and then temperatures from the corresponding thermocouples were overlayed. Analysis revealed complete PANC-1 cell destruction was attained following exposure to a temperature of ~−20 °C or colder.