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. 2024 Feb 23;12(3):507. doi: 10.3390/biomedicines12030507

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Gross pathology of a resected porcine liver tissue following freezing. Following an EUS-based cryoablation procedure using FrostBite, porcine livers were resected and analyzed. Gross pathology of the tissue revealed the consistent creation of ~2 cm × 3.2 cm lesions (deep purple tissue) following a single 3 min freeze (A,B (right lesion) and C). A double 3 min freeze resulted in an increase in the lesion size to ~2.5 cm × 3.5 cm (B, left lesion). Analysis of a freeze lesion created near a blood vessel (D) confirmed the EUS imaging of the cryolesion sculpting around the blood vessel, and the vessel wall showed no obvious signs of damage.