Table 2.
Freeze Protocol (mins) | Single (First) Freeze Isotherm Dia (mm(±SD)) | Repeat (Second) Freeze Isotherm Dia (mm(±SD)) | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
0 °C | −10 °C | −20 °C | −30 °C | −40 °C | 0 °C | −10 °C | −20 °C | −30 °C | −40 °C | |
Single 3 | 16.4 (0.5) | 13.9 (0.4) | 11.6 (0.5) | 9.6 (0.3) | 8.5 (0.3) | |||||
Single 5 | 21.2 (0.7) | 17.8 (0.4) | 14.8 (0.4) | 12.1 (0.4) | 9.7 (0.2) | |||||
Double 3/5/3 | 16.8 (0.6) | 14.2 (0.5) | 12.0 (0.5) | 9.8 (0.4) | 8.6 (0.2) | 20.0 (0.4) | 17.0 (0.7) | 14.0 (0.6) | 11.6 (0.5) | 9.6 (0.4) |
Double 5/5/5 | 22.2 (0.7) | 18.4 (0.6) | 15.2 (0.4) | 12.4 (0.5) | 9.8 (0.2) | 28.2 (1.0) | 23.2 (0.9) | 18.6 (0.6) | 15.0 (0.6) | 11.8 (0.4) |