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. 2024 Feb 20;12(3):470. doi: 10.3390/biomedicines12030470

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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NSL-YHJ-2-27 induces apoptosis in MDA-MB-468 cells. (A) Cells were treated with the indicated concentrations of NSL-YHJ-2-27 (0, 1, 2, and 5 µM), collected, and stained with FLICA and propidium iodide, followed by flow cytometry analysis. (B) PCAIs-treated cells, NSL-YHJ-2-27 (0, 1, 2, and 5 µM) or NSL-YHJ-2-62 (10 µM), were analyzed for apoptotic factors by Western blotting, as described in the Methods. Data are representative of three independent experiments. Statistical significance (*, p < 0.05; **, p < 0.01; ***, p < 0.001) was determined by one-way ANOVA with post hoc Dunnett’s test.

NSL-YHJ-2-27 induces apoptosis in MDA-MB-468 cells. (A) Cells were treated with the indicated concentrations of NSL-YHJ-2-27 (0, 1, 2, and 5 µM), collected, and stained with FLICA and propidium iodide, followed by flow cytometry analysis. (B) PCAIs-treated cells, NSL-YHJ-2-27 (0, 1, 2, and 5 µM) or NSL-YHJ-2-62 (10 µM), were analyzed for apoptotic factors by Western blotting, as described in the Methods. Data are representative of three independent experiments. Statistical significance (*, p < 0.05; **, p < 0.01; ***, p < 0.001) was determined by one-way ANOVA with post hoc Dunnett’s test.