Figure 9.

NSL-YHJ-2-27 inhibits breast cancer cell invasion into Matrigel. MDA-MB-231 cells were seeded in 96U round bottom Nunclon Sphera plates and allowed to grow for 48 h. The preformed spheroids were then treated with the indicated concentrations of NSL-YHJ-2-27 (0, 1, 2, and 5 µM), after which they were embedded in a Matrigel containing the corresponding concentrations of NSL-YHJ-2-27 in three replicates. Once the Matrigel solidified, brightfield (BF) images were obtained using a Nikon Ti Eclipse microscope at 4× magnification for 0 h exposure. (A) The spheroids were incubated for another 72 h, after which BF images were again obtained. Scale bar = 100 µm. (B) The area invaded by the cells was measured from the body of the spheroid using the NIS Element software. Statistical significance (***, p < 0.001) was determined by one-way ANOVA with post hoc Dunnett’s test.