Figure 11.

NSL-YHJ-2-27 disrupts the assembly of vinculin, leading to cell shrinkage. Cells were grown in 8-well ibidi µ-slides until adherence. They were then treated with PCAIs for 48 h and analyzed by immunocytochemistry for vinculin localization using anti-rabbit IgG Alexa Fluor 555 conjugate, as described in the Methods. Images were captured using Keyence fluorescence microscope at 40× magnification. Scale bar = 100 µm. (A). White arrows indicate the original ‘footprints’ of dislodged cells. In (B,C), n = 60 to 100 cells were quantified for fluorescence intensity and area using Keyence BZ-X800 analyzer. Data were plotted using GraphPad Prism version 8. Statistical significance (***, p < 0.001) was determined by one-way ANOVA with post hoc Dunnett’s test.