Figure 12.

NSL-YHU-2-27 decreases the level of vinculin in MDA-MB-231 cells. Cells were treated for 48 h with the indicated concentrations of NSL-YHJ-2-27 or 10 μM of the non-farnesylated PCAIs analog NSL-YHJ-2-62. They were then lysed and analyzed by Western blotting for vinculin, as described in the Methods. (A) Western blot images and (B) chemiluminescence plots of bands following quantification using Image Lab software, normalized against GAPDH. Data are representative of three independent experiments. Statistical significance (*, p < 0.05) was determined by one-way ANOVA with post hoc Dunnett’s test.