FIG. 2.

Presence of mutations in immortalized-cell genomic DNA. PCR of immortalized-cell genomic DNA with primers 6555 and 7492 results in a fragment of 937 bp. Lanes 1 to 5, expected digestion pattern of the ACH.wt-derived fragment; digestion with PstI results in bands of 760 and 177 bp, while digestion with SacII results in bands of 561 and 376 bp; there are no XbaI or BglII sites in the fragment; lanes 6 and 7, the ACH.p12^I mutation deletes the PstI site; lanes 8 and 9, the ACH.p12^I/p30^II mutation deletes the SacII site; lanes 10 and 11, the ACH.p30^II mutation inserts an XbaI site at position 7128, resulting in bands of 573 and 386 bp when the DNA is digested with XbaI; lanes 12 to 14, the ACH.p30^II/p13^II mutation inserts the same XbaI site plus a BglII site at position 7286, which results in bands of 753 and 206 bp when the DNA is digested with BglII.