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IL-2 dependence of mutant-immortalized cell lines. Fifty thousand ACH.wt-, ACH.p12I-, ACH.p12I/p30II-immortalized cells were seeded in triplicate in a 96-well microtiter plate in twofold dilutions of IL-2. Relative proliferation was determined after 72 h in culture by MTT conversion assays. Error bars indicate standard deviations.
