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Viral neutralization assay. Serum to be tested is serially diluted and incubated with a defined quantity of viral particles (1). The virus-serum solutions are added to Vero or BHK cells (2) and incubated for 3/5 days at 37 °C in a humidified atmosphere with 5% CO2. Finally, the cytopathic effect of viral infection is evaluated through microscopic observation (3). Created with Biorender.com.
