Figure 5.

Schematic representation of RVFV isolation. The blood or serum sample is collected from infected hosts (1) and inoculated in cell culture (2). After incubation at 28 °C (AP61 cells) or 37 °C (Vero and BHK cells) for 1 h (3), cell monolayers are washed with PBS and fresh medium is added (4). Virus isolation is validated by RT-PCR analysis (5). Created with Biorender.com.