Table 3.
Stage | Temperature | Time | Cycles |
---|---|---|---|
Initial denaturation | 95 °C | 2 min | 1 cycle |
Denaturation | 95 °C | 30 s | |
Annealing | 60–61 °C | 30 s | 40 cycles |
Termination | 72 °C | 45 s | 1 cycle |
Stage | Temperature | Time | Cycles |
---|---|---|---|
Initial denaturation | 95 °C | 2 min | 1 cycle |
Denaturation | 95 °C | 30 s | |
Annealing | 60–61 °C | 30 s | 40 cycles |
Termination | 72 °C | 45 s | 1 cycle |