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. 2024 Mar 21;12(3):695. doi: 10.3390/biomedicines12030695

Table 3.

The conditions employed for RT-qPCR APOE expression analysis.

Stage Temperature Time Cycles
Initial denaturation 95 °C 2 min 1 cycle
Denaturation 95 °C 30 s
Annealing 60–61 °C 30 s 40 cycles
Termination 72 °C 45 s 1 cycle