Skip to main content
. Author manuscript; available in PMC: 2024 Mar 27.
Published in final edited form as: Nat Struct Mol Biol. 2023 Dec 6;31(1):179–189. doi: 10.1038/s41594-023-01150-0

Extended Data Fig. 3. Cryo-EM data processing and validation.

Extended Data Fig. 3

a. Example micrograph. b. Initial 2D class averages. c. Data processing workflow highlighting all relevant steps. d. Fourier Shell Correlation (FSC) curves for cross-validation between the map and model of the pre-branching group II intron. e. Euler angle distribution plot showing the orientations of particles used in the reconstruction. f. Local resolution map of the pre-branching group II intron map (map made in UCSF Chimera). g. Surface sampling plot of the Fourier sampling, with SCF value shown. h. 3D FSC shown as an isosurface at a threshold of 0.5 with three perpendicular planar views.