Cryo-EM data collection, refinement and validation statistics
| Pre-branching group IIB intron (EMDB-29279) (PDB 8FLI) | |
|---|---|
|
| |
| Data collection and processing | |
| Magnification | 45,000x |
| Voltage (kV) | 200 |
| Electron exposure (e–/Å2) | 31.6 |
| Defocus range (μm) | 1.0 – 2.5 |
| Pixel size (Å) | 0.92 |
| Symmetry imposed | No |
| Initial particle images (no.) | 476,053 |
| Final particle images (no.) | 38,881 |
| Map resolution (Å) | 4.0 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.5–14.5 |
| Refinement | |
| Initial model used (PDB code) | 6MEC |
| Model resolution (Å) | 3.8 |
| FSC threshold | 0.143 |
| Model resolution range (Å) | |
| Map sharpening B factor (Å2) | −100.9 |
| Model composition | |
| Non-hydrogen atoms | 21511 |
| Protein residues | 458 |
| RNA residues | 828 |
| Ligands | 3 |
| B factors (Å2) | |
| Protein | 75.13 |
| RNA | 154.21 |
| Ligand | 94.21 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 0.678 |
| Validation | |
| MolProbity score | 2.39 |
| Clashscore | 15.68 |
| Poor rotamers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 82.89 |
| Allowed (%) | 16.45 |
| Disallowed (%) | 0.66 |