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Echinatin inhibits sevoflurane-induced ferroptosis in HT22 cells. HT22 cells were subjected to Fer-1 (1 μM) treatment or Echinatin (40μM) treatment for 24 hours, followed by exposure to sevoflurane or control conditions. (A) Cell apoptosis was measured by flow cytometry. (B) Intracellular Fe2+ detected by FerroOrange. (C) Protein levels of FPN, TFR1, and DMT1 were measured by western blot. The data are presented as the mean ± SD. Compared with the Sevo group, *P<0.05, **P<0.01.
