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Inactive carboxypeptidase-like protein X2 (CPXM2) ELISA validation. (a) Box plots of direct homemade ELISA assay for CPXM2 protein in unprocessed total fraction (Tot) of EVD CSF obtained from congenital hydrocephalus (CH), pilocytic astrocytoma (PA), and medulloblastoma (MB) patients. CPXM2 is statistically more abundant in PA (p < 0.0001) samples compared to CH and MB samples. (b) ROC curve analysis for the CPXM2 ELISA assay.
