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. 2024 Mar 8;13(6):481. doi: 10.3390/cells13060481

Figure 3.

Figure 3

Indoxyl sulfate induces inflammation and a decrease in ILK levels in hVECs. hVECs were treated with IS (250 μM) (A) Immunofluorescence representation of NF-kB (green) translocation to nuclei (Hoechst blue) in cells treated with IS for 8 and 24 h; (B) RT-qPCR of CT- and IS-treated hVEC RNA extracts showing mRNA expression of VCAM-1 (** p < 0.001; n = 3); (C) RT-qPCR of CT- and IS-treated hVEC RNA extracts showing mRNA expression of ILK (*** p < 0.0001; n = 3); (D) Western blot analysis and quantification of ILK in hVECs treated with IS or CT for 3–7 days (** p < 0.001; n = 3). Black bar (CT) and red bars (IS). Experiments were performed in duplicate.