FIG. 2.

Absence of interaction of Tip mSH3B with Lck in transformed T cells. A total of 2 × 10⁷ cells were the source of extracts for immunoprecipitations with anti-Tip (α Tip) and anti-Lck (α Lck) antibodies. Each immune complex was subjected to in vitro kinase assay with [γ-³²P]ATP. Lane 1, IL-2-stimulated common marmoset PBMCs; lane 2, wt HVS-transformed common marmoset T cells; lane 3, HVS/Tip mSH3B-transformed common marmoset T cells. Top arrows indicate Lck; bottom arrows indicate Tip; sizes are indicated in kilodaltons.