Figure 5.

Immunofluorescence analysis of vimentin expression in response to CB1-agonists. CB1-agonists, AM-404, Arvanil, and Olvanil, significantly increased the expression of the epithelial–mesenchymal transition (EMT), vimentin, in SW-620 cells. Cells were fixed, permeabilized, blocked, and immunostained with primary mouse monoclonal IgG antibodies for vimentin, followed by goat anti-mouse AlexaFluor 488 (green) secondary antibodies. As an isotype control, we use normal mouse IgG. The fluorescence was calculated using Corel Photo-Paint, with the average of eight points subtracted by the background fluorescence and multiplied by the pixel density (20× objective). Statistical significance, as indicated by asterisks, was calculated with ANOVA and Fisher’s uncorrected LSD multiple comparisons post hoc test at a confidence level of 95%. ns = non-significant, **** p < 0.0001, *** p < 0.001.