Figure 1.

A model of experimental autoimmune myasthenia gravis. (A): Experimental autoimmune myasthenia gravis (EAMG) score scored weekly. Mice were immunized in week 8 and 12 with n = 12 per group. EAMG mice were immunized with CHRNA1 antigen and complete Freund’s adjuvant. Controls received only complete Freund’s adjuvant. (B): Mean EAMG score of weeks 12 to 18 for each group. (C): Forelimb grip strength was scored biweekly after exercising the animals. (D): Mean forelimb grip strength of weeks 12 to 18 for each group. Antibodies were quantified by enzyme-linked immunosorbent assay. (E): Immunofluorescence staining of representative muscle specimen of EAMG and control mice. α-bungarotoxin (α-bung) binds the acetylcholine receptor (red). Complement deposits are stained with C3 (green or yellow) and IgG (blue). Groups were compared by unpaired student’s t-test., p < 0.01 **, p < 0.001 ***, p > 0.05 not significant.