Figure 3.

EAMG mice demonstrate a reduced number of NMJs. (A): Left: Immunofluorescence staining of representative muscle specimen of EAMG and control mice. α-bungarotoxin (α-bun) binds the acetylcholine receptor (red) while neurofilament light chain (NFL) binds to peripheral nerves (green). Right: Zoom in on exemplary NMJs highlighting a loss of topographic complexity in EAMG mice. (B): NMJs were counted in randomly distributed 10 HPF (≙0.16 mm²). The biopsies were blinded for quantification, with the group impossible to identify from the label. Groups were compared by unpaired Student’s t-test. p ≤ p < 0.01 **. Abbreviations: EAMG, experimental autoimmune myasthenia gravis; HPF, high-power field.