Figure 6.

Relative mRNA expression levels of the DACH1 gene during megakaryocytic differentiation process in the CD34–MK in vitro differentiation model. (A) Dynamic change in the percentages of CD61⁺ and CD61⁺CD42b⁺ cells in the R1 region. Cells were stained with antibodies against CD61 and CD42b and analyzed using flow cytometry on Days 0, 4, 8, 12, and 16. (B) Relative mRNA expression levels of the DACH1 gene were analyzed using qRT-PCR and normalized to the GAPDH gene. The mRNA expression level of the DACH1 genes in the Day 0 group was considered one-fold. Data are presented as mean ± SEM. * p value < 0.05, ** p value < 0.01, compared with the Day 0 group.