Figure 3.

Effect of cytomix on cell cycle progression in A549 WT and IRF1 KO cells. Cells were incubated in the absence (control) or in the presence of 10 ng/mL IFNγ + 50 ng/mL TNFα. Panel (A): After 24 h of incubation, cells were harvested, stained with propidium iodide and analyzed for cell cycle with flow cytometry, as detailed in Methods. Plots obtained in a representative experiment are shown. Cell distribution in each phase of the cell cycle is also shown (bottom); bars are the mean ± SEM of data obtained in three independent experiments. * p < 0.05, ** p < 0.01 vs. control with Student’s t-test. Panel (B): At the indicated times, the expression of the indicated proteins was assessed by means of Western Blot analysis, as detailed in Methods. Representative blots are shown along with the mean ± SEM of the densitometric analysis of three different experiments. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. 0 h (untreated cells) with One-way ANOVA.