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. 2024 Mar 19;25(6):3448. doi: 10.3390/ijms25063448

Figure 2.

Figure 2

Effects of oral treatment with ED or its ingredient, Trp, on the induction of Treg differentiation in the splenocytes and serum Kyn concentrations in mice. C57BL/6 mice were orally treated with distilled water (vehicle control), 5 mL/day of ED and 1, 10, or 100 mg/kg of Trp, and sacrificed 24 h after administration. (a) Body weight (g) 24 h after administration. (b,c) Treg induction in each individual mouse spleen was measured by flow cytometry. (d) Serum Kyn concentrations (µM) were determined by LC-MS/MS 24 h after dosing. Values represent the means ± SD of five animals. * p < 0.05 vs. the vehicle control (leftmost bar), by (a,b,d) t-test, (c) Dunnet’s test.