Figure 4.
Effects of Trp metabolites on transcriptional activity via AhR and in silico docking analysis. (a) The metabolic pathways of tryptophan and Trp metabolites used in this study. (b) AhR-mediated transcriptional activity of active 5 Trp metabolites in DR-EcoScreen cell assay. The control was added at 0.1% DMSO, TCDD at 1 × 10−10 M, and other compounds at concentrations of ≤3 × 10−4 M. (c) Results of in silico docking simulation of TCDD, Kyn, tryptamine, and 3-methylindole against mouse AhR (PDB code 4M4X) using Molegro Virtual Docker. White arrows indicate docking sites.