Table 2.

Significant differentially methylated regions (DMRs) in second-trimester maternal blood specimens comparing high vs. low BTEX exposure. DMRs are arranged by increasing FDR p-value.

						CpG Sites *
Chromosome	BP Start	BP Width	Functional Annotation	Gene Symbol	FDR	Total	Hyper	Hypo
4	184,908,253	766	Distal intergenic	LINC01093	1.67 × 10−9	11	0	10
6	291,686	911	Promoter (≤1 kb)	DUSP22	1.76 × 10−9	8	0	8
11	6,291,624	888	Distal intergenic	CCKBR	1.76 × 10−9	7	0	7
17	40,274,523	289	Promoter (≤1 kb)	WIPF2	1.02 × 10−7	7	0	6
1	42,384,283	365	Intron	RIMKLA	2.23 × 10−7	8	0	8
7	69,064,092	87	Distal intergenic	CT66	3.26 × 10−7	4	0	4
2	48,132,739	315	Intron	FOXN2	3.26 × 10−7	7	0	7
17	80,408,535	394	Intron	RNF213-AS1	3.30 × 10−7	8	0	6
21	47,532,059	275	Distal intergenic	PRMT2	6.89 × 10−7	5	0	4
12	123,319,893	165	Exon	SBNO1	8.02 × 10−7	5	0	4
14	93,698,773	172	Intron	UNC79	8.02 × 10−7	3	0	3
11	123,430,574	376	Promoter (≤1 kb)	GRAMD1B	8.67 × 10−7	6	0	6
1	161,008,461	366	Intron	F11R	8.90 × 10−7	8	3	3
12	123,750,781	84	Promoter (≤1 kb)	ATP6V0A2	9.13 × 10−7	3	0	3
5	170,814,528	309	Distal intergenic	GABRP	9.13 × 10−7	8	0	8
19	2,163,592	241	Promoter (≤1 kb)	DOT1L	1.01 × 10−6	4	0	4
11	9,697,192	238	Intron	SWAP70	1.03 × 10−6	2	1	1
17	53,828,262	254	Intron	KIF2B	1.03 × 10−6	6	0	4
19	35,645,555	158	Promoter (1–2 kb)	ETV2	1.03 × 10−6	8	0	6
16	56,228,384	361	Promoter (2–3 kb)	GNAO1	1.87 × 10−6	9	0	7
3	9,932,179	145	Promoter (1–2 kb)	IL17RC	1.93 × 10−6	6	0	4
2	48,844,762	307	Intron	STON1-GTF2A1L	2.31 × 10−6	6	0	5
22	44,422,011	189	Distal intergenic	LINC01656	2.46 × 10−6	5	3	2
16	10,837,596	110	Distal intergenic	TVP23A	2.71 × 10−6	8	0	6
6	85,823,948	269	Distal intergenic	SNHG5	4.21 × 10−6	4	0	4
11	117,352,729	211	Promoter (≤1 kb)	CEP164	5.14 × 10−6	4	0	4
14	105,287,325	103	Intron	BRF1	5.46 × 10−6	3	0	3
19	37,825,319	254	Promoter (2–3 kb)	LOC644554	6.11 × 10−6	6	0	6
10	133,938,603	291	Distal intergenic	FRG2B	6.18 × 10−6	5	0	3
3	15,469,026	292	Intron	COLQ	8.95 × 10−6	6	0	4
19	49,222,966	288	Distal intergenic	TRPM4	1.16 × 10−5	4	0	3
11	116,658,839	232	Distal intergenic	LINC02702	3.16 × 10−5	5	0	5
7	95,064,396	79	Intron	PPP1R9A	3.97 × 10−5	5	0	4
16	1,199,498	27	Intron	CACNA1H	4.70 × 10−5	2	0	2
9	130,533,824	27	Distal intergenic	FUBP3	6.41 × 10−5	4	0	2
16	1,031,442	17	Distal intergenic	SSTR5-AS1	7.07 × 10−5	2	0	2
16	66,638,395	205	Promoter (1–2 kb)	CMTM4	1.27 × 10−4	6	0	4
16	12,070,415	274	Intron	SNX29	1.42 × 10−4	5	0	4
19	44,259,187	10	Promoter (≤1 kb)	ZNF233	1.44 × 10−4	2	0	2
10	101,282,815	69	Distal intergenic	LINC02681	2.29 × 10−4	2	2	0
12	111,126,997	143	Intron	CUX2	6.16 × 10−4	4	1	1
1	166,958,580	4	Intron	ILDR2	0.001	2	0	2
3	88,198,600	152	Distal intergenic	C3orf38	0.002	4	0	1
11	368,564	75	Promoter (≤1 kb)	B4GALNT4	0.003	5	0	2
2	8,597,158	31	Distal intergenic	LINC01814	0.003	3	0	2
2	1846836	18	Intron	MYT1L	0.004	2	0	2

BP, base pair; BTEX, benzene, toluene, ethylbenzene, and xylenes; FDR, false discovery rate. BP Start denotes the 5’ most base pair position within each region based on genome build hg38. BP Width is the number of base pairs over which each region spans. Functional Annotation corresponds to the annotation category assigned to each region by CHiPseeker. Gene Symbol is the official gene symbol for the gene nearest to the region identified by CHiPseeker. False discovery rate is the adjusted p-value for the region as determined by comb-p. * The total number of CpG sites within each DMR, and the subset with single CpG site nominal p-values < 0.05, where increasing BTEX exposure was associated with hyper- and hypo-methylation.