Figure 6.

Whole-mount in situ hybridization of skeletal myogenesis marker myod in control and knockout zebrafish embryos at 10, 24, and 36 hpf. (a) Whole-mount in situ hybridization of skeletal myogenesis marker myod in non-injected (NI) embryos at 10, 24, and 36 hpf. (b) Whole-mount in situ hybridization of skeletal myogenesis marker myod in only Cas9-injected embryos at 10, 24, and 36 hpf. (c) Whole-mount in situ hybridization of skeletal myogenesis marker myod in knockout embryos (KO) at 10, 24, and 36 hpf. Arrows indicate the abnormal expression of myod in myotomes. NI indicates no injection. KO indicates knockout. Microscope’s magnification: 45 ×