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. 2024 Mar 16;25(6):3379. doi: 10.3390/ijms25063379

Figure 2.

Figure 2

Western blot analysis showing the concentration-dependent induction of claudin-5 (Cld-5) expression by E2 and DPN. The endothelial cell lines cEND and hCMEC/D3 were analyzed for 24 h in the concentrations 10−12, 10−10, 10−8, and 10−6 M with E2 or DPN. (a) E2-dependent Cld-5 expression of the murine endothelial cell line cEND; (b) DPN-dependent Cld-5 expression of the murine endothelial cell line cEND; (c) E2-dependent Cld-5 expression of the human endothelial cell line hCMEC/D3; (d) DPN-dependent Cld-5 expression of the human endothelial cell line hCMEC/D3; (e) Representative bands of the protein expression patterns of claudin-5 (Cld-5) in cEND and hCMEC/D3 after differentiation with E2 or DPN at the concentrations described. All means ± SD; n = 3; Shapiro–Wilk test (α = 0.05): passed Dunnett’s post hoc multiple comparisons test; 0.1234 (ns), 0.0332 (*).