FIG. 1.

Schematic presentation of IBDV cDNA constructs for the generation of plus-sense RNA transcripts with T7 RNA polymerase. Plasmid pUC19FLAD78 encodes the polyprotein (VP2-VP4-VP3) and the NS protein (NS) of D78-IBDV. In plasmid pUCD78NSΔ, the initiation codon of NS gene is mutated to a stop codon. Plasmid pUCD78B encodes the RNA-dependent RNA polymerase (VP1). All plasmids contain a T7 promoter sequence at their 5′-ends. Plasmids were linearized with the appropriate restriction enzymes as indicated (↑).