TABLE 3.
Detection of virus in the BF of chickens infected with rD78 or rD78NSΔ IBDV
| Group | Virus isolationa on day:
|
RT-PCRb on day:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 4 | 6 | 9 | 21 | 2 | 4 | 6 | 9 | 21 | |
| Control | − | − | − | − | − | − | − | − | − | − |
| rD78 | + | + | + | − | − | + | + | + | − | − |
| rD78NSΔ | + | + | + | + | − | + | + | + | + | − |
a
BF from each group were pooled, and bursal homogenates were used to inoculate secondary CEF cells. +, presence of cytopathic effect; −, absence of cytopathic effect. The virus titers of each group are shown in Table 4.
b
+, virus detected by RT-PCR; −, no virus detected by RT-PCR.